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Multiple drug resistance patterns and plasmid profiles of non-typhi salmonellae in Turkey

T Yildirmak1, A Yazgan, G Ozcengiz

  • 1Ankara Numune Hospital, Department of Infectious Diseases and Clinical Microbiology, Turkey.

Epidemiology and Infection
|November 24, 1998
PubMed

Insights

A study of 259 non-typhi salmonellae (NTS) isolates found 19.3% were multi-drug resistant (MDR). MDR strains, primarily S. typhimurium, showed diverse antibiotic resistance patterns and plasmid profiles, with no direct correlation observed.

Area of Science:

  • Microbiology
  • Molecular Biology
  • Public Health

Background:

  • Non-typhi Salmonellae (NTS) are significant human pathogens.
  • Antibiotic resistance in NTS poses a growing public health threat.
  • Understanding resistance mechanisms in NTS is crucial for effective treatment.

Purpose of the Study:

  • To investigate antibiotic resistance patterns in clinical isolates of NTS.
  • To determine the prevalence and characteristics of plasmids in multi-drug resistant (MDR) NTS strains.
  • To explore the correlation between antibiotic resistance and plasmid content in NTS.

Main Methods:

  • Analysis of 259 clinical NTS isolates for antibiotic susceptibility testing against 17 antibiotics.
  • Identification of multi-drug resistant (MDR) strains and their resistance patterns.
  • Plasmid DNA extraction, profiling, and size determination for MDR isolates.

Main Results:

  • 19.3% (50/259) of NTS isolates exhibited multi-drug resistance (MDR).
  • MDR strains were predominantly Salmonella Typhimurium (49/50).
  • Fifteen distinct antibiotic resistance patterns were observed; 28 MDR isolates carried plasmids (mean 5 plasmids), with heterogeneous plasmid profiles (21 types) and sizes ranging from 1.7 to 158 kb. No direct correlation was found between resistance patterns and plasmid profiles.

Conclusions:

  • A significant proportion of NTS clinical isolates are MDR, with Salmonella Typhimurium being a major contributor.
  • MDR NTS strains harbor diverse plasmids, but a direct link between specific resistance patterns and plasmid profiles was not established in this study.
  • Further research is needed to elucidate the genetic basis of antibiotic resistance in NTS and its epidemiological implications.

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