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Related Experiment Videos

Connexins in the developing salivary glands

M Shimono1, T Muramatsu, A Ihara

  • 1Oral Health Science Center, and Dept. Pathology, Tokyo Dental College, Chiba, Japan.

European Journal of Morphology
|November 24, 1998
PubMed
Summary

Connexins (Cx43 and Cx32) were studied in developing rat submandibular glands. Cx43 was present early, suggesting a role in gland growth, while Cx32 increased post-birth, potentially regulating secretion.

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Area of Science:

  • Cell Biology
  • Developmental Biology
  • Histology

Background:

  • Gap junctions are crucial for intercellular communication and tissue development.
  • Connexins (Cx32 and Cx43) are the protein components of gap junctions.
  • The role of specific connexins in the development of exocrine glands like the submandibular gland is not fully understood.

Purpose of the Study:

  • To investigate the ultrastructure of gap junctions and the expression patterns of connexins Cx32 and Cx43 during the development of rat submandibular glands.
  • To correlate the presence and distribution of Cx43 and Cx32 with specific developmental stages, from embryonic to postnatal periods.

Main Methods:

  • Utilized freeze-fracture electron microscopy to visualize gap junction ultrastructure.
  • Employed immunohistochemistry to detect and localize Cx43 and Cx32 expression in glandular cells.

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  • Examined tissue samples from the 17th gestational day to the 14th day after birth.

    • Main Results:

    • Gap junctions were observed as small clusters of particles during gestation, with specific dimensions and spacing.
    • Cx43 expression was detected early (from the 17th gestational day) in glandular cells.
    • Cx32 expression was minimal during gestation but increased significantly after birth.

    Conclusions:

    • Cx43 expression appears to be associated with the prenatal growth and differentiation of rat submandibular glands.
    • Cx32 expression, increasing postnatally, may play a role in establishing secretory regulation in these glands.
    • Differential expression of connexins suggests distinct roles in gland development and function.