Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

TNFa and b microsatellites in Germany

E Müller1, K H Frank, M Füssel

  • 1Institut für Rechtsmedizin, Universitätsklinikum der Technischen Universität Dresden, Germany.

International Journal of Legal Medicine
|November 24, 1998
PubMed
Summary

Tumor Necrosis Factor (TNF) microsatellite allele frequencies were analyzed in healthy Germans. These microsatellites showed stable inheritance and Mendelian segregation, with no new mutations detected.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Hospitalization for vitamin-K-antagonist-related bleeding: treatment patterns and outcome.

Journal of thrombosis and haemostasis : JTH·2013
Same author

Desmopressin testing in haemophilia A patients and carriers: results of a multi centre survey.

Hamostaseologie·2012
Same author

Efficacy and safety of rivaroxaban or fondaparinux thromboprophylaxis in major orthopedic surgery: findings from the ORTHO-TEP registry.

Journal of thrombosis and haemostasis : JTH·2012
Same author

Intragastric acidity during administration of generic omeprazole or esomeprazole - a randomised, two-way crossover study including CYP2C19 genotyping.

Alimentary pharmacology & therapeutics·2010
Same author

Central adjudication of venous ultrasound in VTE screening trials: reasons for failure.

Journal of thrombosis and haemostasis : JTH·2010
Same author

Impaired insulin turnover in islets from type 2 diabetic patients.

Islets·2010

Area of Science:

  • Human genetics
  • Molecular biology
  • Population genetics

Background:

  • Microsatellites are polymorphic DNA regions crucial for genetic studies.
  • Tumor Necrosis Factor (TNF) gene region microsatellites are of interest for population genetics and disease association studies.
  • Understanding the inheritance patterns of these microsatellites is fundamental for their application in genetic research.

Purpose of the Study:

  • To determine the allele frequencies of Tumor Necrosis Factor alpha (TNFa) and Tumor Necrosis Factor beta (TNFb) microsatellites in a healthy German population.
  • To investigate the inheritance patterns and segregation of these microsatellites.
  • To assess the occurrence of new mutations in these specific microsatellite loci.

Main Methods:

  • Polymerase Chain Reaction (PCR) was employed to amplify TNFa and TNFb microsatellite regions.

Related Experiment Videos

  • DNA samples from 315 healthy, unrelated German individuals (including mothers and putative fathers) were analyzed.
  • Microsatellite alleles were identified and quantified to determine frequencies.

    • Main Results:

    • Allele frequencies for TNFa and TNFb microsatellites were successfully determined in the studied German cohort.
    • The microsatellites demonstrated stable inheritance patterns across generations.
    • Segregation analysis confirmed adherence to Mendelian inheritance principles.
    • No new mutations were observed at the analyzed microsatellite loci.

    Conclusions:

    • The TNFa and TNFb microsatellites are reliably inherited in a Mendelian fashion within the German population.
    • These microsatellites exhibit stability, making them suitable markers for population genetic studies.
    • The absence of new mutations suggests their utility for genetic analysis without significant confounding factors.