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Sequence variation of a variable short tandem repeat at the D18S535 locus
1Institute of Legal Medicine, Faculty of Medicine, Santiago de Compostela, Spain.
International Journal of Legal Medicine
|November 24, 1998
Summary
Researchers analyzed a short tandem repeat (STR) at the D18S535 locus, finding 8 distinct alleles. This highly polymorphic DNA marker is suitable for forensic and genetic applications.
Area of Science:
- Genetics
- Forensic Science
- Molecular Biology
Background:
- Short tandem repeats (STRs) are valuable genetic markers.
- The D18S535 locus contains a specific STR with potential forensic applications.
Purpose of the Study:
- To sequence and characterize alleles at the D18S535 locus.
- To evaluate the polymorphic nature and suitability of this STR for forensic and genetic analyses.
Main Methods:
- Sequencing of 25 selected alleles from the D18S535 locus.
- Analysis of repeat structure and allele designation based on repeat number.
Main Results:
- Identified 8 different alleles at the D18S535 locus.
- Characterized the STR as a simple hypervariable repeat with a (GATA)n motif.
- Determined a high heterozygosity rate (>0.80) and small amplicon size (130-158 bp).
Conclusions:
- The D18S535 STR is highly polymorphic and possesses a simple structure.
- Its characteristics make it a promising DNA marker for forensic identification and genetic studies.