Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

DNA end-joining catalyzed by human cell-free extracts

P Baumann1, S C West

  • 1Imperial Cancer Research Fund, Clare Hall Laboratories, South Mimms, Herts EN6 3LD, United Kingdom.

Proceedings of the National Academy of Sciences of the United States of America
|November 25, 1998
PubMed
Summary

This study developed an in vitro system to study DNA end-joining in human cells. This system revealed the key proteins involved in accurate DNA repair and V(D)J recombination.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Correction: Non-Response to Antidepressants: Risk Factors and Therapeutic Possibilities.

Pharmacopsychiatry·2026
Same author

Dynamic allele usage of X-linked genes ameliorates neurodevelopmental disease phenotypes in brain organoids.

Nature communications·2026
Same author

Three-year follow-up analysis of the short-stitch versus long-stitch technique for elective midline abdominal closure randomized-controlled (ESTOIH) trial.

Hernia : the journal of hernias and abdominal wall surgery·2024
Same author

Prospective, multi-centric, international, single-arm, cohort study to assess a synthetic polyamide suture material in oral surgery to close the mucosa - MUCODA study.

Journal of dentistry·2024
Same author

The 6:1 short stitch SL-WL-ratio: short term closure results of transverse and midline incisions in elective and emergency operations.

Hernia : the journal of hernias and abdominal wall surgery·2024
Same author

Effects of the short-stitch technique for midline abdominal closure: short-term results from the randomised-controlled ESTOIH trial.

Hernia : the journal of hernias and abdominal wall surgery·2021

Area of Science:

  • Molecular Biology
  • Genetics
  • Biochemistry

Background:

  • Mammalian cells lacking DNA end-joining capabilities exhibit extreme sensitivity to ionizing radiation.
  • Defective DNA end-joining leads to immunodeficiency due to incomplete V(D)J recombination.

Purpose of the Study:

  • To develop an in vitro system for studying DNA end-joining in human cells.
  • To identify the molecular components essential for accurate DNA end-joining.

Main Methods:

  • Utilized cell-free extracts from human lymphoblastoid cell lines.
  • Established an in vitro system to analyze intermolecular DNA ligation.

Main Results:

  • The in vitro system demonstrated accurate intermolecular ligation.

Related Experiment Videos

  • DNA ligation was found to be dependent on DNA ligase IV/Xrcc4 complex.
  • The process requires Ku70, Ku86, and DNA-PKcs, subunits of DNA-activated protein kinase DNA-PK.

    • Conclusions:

    • The developed in vitro system is crucial for understanding DNA end-joining mechanisms in human cells.
    • Identified key proteins involved in DNA repair, radiation resistance, and V(D)J recombination.