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Streptomyces species isolated from potato scabs in France: numerical analysis of "Biotype-100" carbon source
K Bouchek-Mechiche1, C Guérin, B Jouan
1INRA, Station de Pathologie Végétale, Le Rheu, France.
Abstract:
Thirty one strains of Streptomyces isolated from common and netted scabs in France were characterized using "Biotype-100" strips and 14 conventional tests. Numerical analysis showed that S. scabiei was the single causal agent of common scab in France. However the strains clustered among S. scabiei were phenotypically heterogeneous, since 3 subphenons were delineated. The strains clustered in subphenons 1a and 1c and one strain of subphenon 1b were associated with common scab. On the other hand, a group of strains clustered in phenon 2, distinct from S. scabiei, S. acidiscabies and S. caviscabies, was often associated with symptoms of netted scab. None of the French strains thus far pathogenic to potato belonged to the already described species S. acidiscabies and S. caviscabies from Japan and North America. Phenotypic characteristics clearly differentiated all the various groups of strains described.
Insights
Streptomyces scabiei causes common potato scab in France, but exhibits phenotypic diversity. A distinct Streptomyces group causes netted scab, differing from known species.
Area of Science:
- Plant Pathology
- Microbiology
- Bacteriology
Background:
- Potato scab, a significant agricultural disease, is caused by Streptomyces species.
- Understanding the diversity and pathogenicity of Streptomyces strains is crucial for disease management.
Purpose of the Study:
- To characterize Streptomyces strains isolated from French potato scab lesions.
- To identify the causal agents of common and netted scab in France.
- To assess the phenotypic diversity within S. scabiei and differentiate from other species.
Main Methods:
- Isolation and characterization of 31 Streptomyces strains from potato scabs.
- Utilized "Biotype-100" strips and 14 conventional biochemical tests.
- Employed numerical analysis for strain clustering and differentiation.
Main Results:
- Streptomyces scabiei was identified as the sole agent of common scab in France.
- French S. scabiei strains displayed significant phenotypic heterogeneity, forming three subphenons.
- A distinct phenon (phenon 2) was associated with netted scab symptoms, separate from S. scabiei, S. acidiscabies, and S. caviscabies.
- No French pathogenic strains belonged to the described species S. acidiscabies or S. caviscabies.
Conclusions:
- S. scabiei is the primary cause of common potato scab in France, with notable phenotypic variations.
- A novel Streptomyces group, distinct from known species, is implicated in netted scab.
- Phenotypic characterization effectively differentiates these potato scab-associated Streptomyces groups.
