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Bioactive substances in buffy-coat-derived platelet pools stored in platelet-additive solutions
L Edvardsen1, E Taaning, T Mynster
1Surgical Immunology Laboratory, Hvidovre University Hospital, Denmark.
British Journal of Haematology
|November 25, 1998
Summary
Platelet additive solutions (PAS) contain bioactive substances like histamine, PAI-1, and MPO upon preparation. Storage of buffy-coat-derived platelets in PAS showed increased PAI-1 levels over time.
Area of Science:
- Transfusion Medicine
- Hematology
- Biochemistry
Background:
- Platelet additive solutions (PAS) are used to store buffy-coat-derived platelet pools.
- Understanding the extracellular accumulation of bioactive substances in platelets is crucial for transfusion safety.
Purpose of the Study:
- To investigate the extracellular accumulation of histamine, plasminogen activator inhibitor 1 (PAI-1), myeloperoxidase (MPO), and interleukin-6 (IL-6) in buffy-coat-derived platelet pools stored in PAS.
- To assess changes in these substances during storage.
Main Methods:
- Preparation of 20 buffy-coat-derived platelet pools in PAS I or PAS II.
- Measurement of histamine, PAI-1, MPO, and IL-6 in donor samples and platelet pools at days 0, 5, and 7.
- Statistical analysis to determine significant differences.
Main Results:
- Significant extracellular levels of histamine, PAI-1, and MPO were detected immediately after platelet pool preparation.
- PAI-1 levels significantly increased during storage from day 0 to day 7.
- IL-6 was detected in only a small fraction of platelet pools, despite buffy-coat preparation aimed at reducing leukocytes.
Conclusions:
- Buffy-coat-derived platelet pools stored in PAS contain significant levels of bioactive substances upon preparation.
- PAI-1 accumulation increases during storage, potentially impacting platelet quality.
- Even with low-leukocyte preparation methods, contamination with other bioactive substances can occur.