Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Fully integrated biocatalytic electrodes based on bioaffinity interactions

E Katz1, V Heleg-Shabtai, A Bardea

  • 1Institute of Chemistry, Hebrew University of Jerusalem, Israel. ekatz@vms.huji.ac.il

Biosensors & Bioelectronics
|November 26, 1998
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Coherent electronic and nuclear dynamics in a rhodamine heterodimer-DNA supramolecular complex.

Physical chemistry chemical physics : PCCP·2017
Same author

Continuous variables logic <i>via</i> coupled automata using a DNAzyme cascade with feedback.

Chemical science·2017
Same author

Electrostatically stabilized hybrids of carbon and maghemite nanoparticles: electrochemical study and application.

Physical chemistry chemical physics : PCCP·2017
Same author

The prevalence of obstructive sleep apnea in symptomatic patients with syndromic craniosynostosis.

International journal of oral and maxillofacial surgery·2015
Same author

Neurocognitive status is associated with all-cause mortality among psychiatric, high-risk liver transplant candidates and recipients.

International journal of psychiatry in medicine·2015
Same author

Enzyme logic AND-Reset and OR-Reset gates based on a field-effect electronic transducer modified with multi-enzyme membrane.

Chemical communications (Cambridge, England)·2015
Same journal

AI-driven photophysics-aware design of fluorescent probes with applications in α-synuclein biosensing and inhibitor screening.

Biosensors & bioelectronics·2026
Same journal

Three-dimensional helical integration of high-density linear microelectrode arrays and their cross-tissue applications.

Biosensors & bioelectronics·2026
Same journal

Integration of electrochemical sensors in organ-on-a-chip microfluidic platforms: Advances and perspectives.

Biosensors & bioelectronics·2026
Same journal

DNN-PURE: A deep neural network approach to paper-based urea sensing.

Biosensors & bioelectronics·2026
Same journal

Rationally architected MOF-derived Co<sub>3</sub>O<sub>4</sub>@NiMn-LDH hollow heterostructure-based sensor array empowering sensitive detection and discrimination of neurological biomarkers.

Biosensors & bioelectronics·2026
Same journal

Four-in-one multifunctional CoCu-NC@AuPt nanozyme integrated M13 phage-displayed nanobody based multimodal lateral flow immunoassay for bovine lactoferrin detection.

Biosensors & bioelectronics·2026
See all related articles

Researchers developed novel bioelectrodes by immobilizing enzymes on electrically contacted surfaces. These integrated systems demonstrate efficient bioelectrocatalysis for lactate oxidation and nitrate reduction, paving the way for advanced biosensors.

Area of Science:

  • Bioelectrochemistry
  • Enzyme immobilization
  • Biosensor development

Background:

  • Enzyme immobilization on electrode surfaces is crucial for developing stable and efficient bioelectrocatalytic systems.
  • Designing affinity matrices that facilitate enzyme binding and cross-linking is key to creating integrated bioelectrodes.

Purpose of the Study:

  • To construct integrated, electrically contacted bioelectrodes using affinity matrices for enzyme immobilization.
  • To demonstrate the bioelectrocatalytic activity of immobilized enzymes for specific redox reactions.

Main Methods:

  • Assembly of enzymes onto electrically contacted affinity matrices (e.g., pyrroloquinoline quinone-NAD+ dyad, microperoxidase-11).
  • Utilizing temporary affinity binding followed by cross-linking to form stable enzyme monolayers.

Related Experiment Videos

  • Characterization of bioelectrocatalytic activity for lactate oxidation and nitrate reduction.

    • Main Results:

    • Successfully assembled bioelectrodes with NAD+-dependent lactate dehydrogenase (LDH) showing catalytic oxidation of lactate.
    • Developed electrodes with nitrate reductase (NR) and myoglobin (Mb) exhibiting catalytic nitrate reduction and hydrogenation.
    • Demonstrated vectorial electron transfer in synthetic heme-protein monolayers.

    Conclusions:

    • Integrated bioelectrodes with cross-linked enzyme monolayers can be efficiently constructed using affinity matrices.
    • These bioelectrodes exhibit significant bioelectrocatalytic activity, applicable to various redox processes.
    • The developed methods offer a versatile platform for advanced biosensor and bioelectrocatalytic device fabrication.