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Expression of a fusion protein of scFv-biotin mimetic peptide for immunoassay
1Research and Development, AltaRex Corp., University of Alberta, Edmonton, Canada.
Journal of Biotechnology
|November 26, 1998
Abstract:
We constructed two fusion proteins of scFv linked to biotin mimetic sequence (BMS) via different linkers, and expressed them in the Pichia pastoris expression/secretion system. We found that both bi-functional scFv proteins exhibited their intrinsic binding activities to antigen CA125 determined in competitive radioimmunoassay experiments, but the fusion protein with a spacer between the scFv and BMS (scFv-spacer-BMS) showed higher binding activity of streptavidin than the one with c-Myc peptide as a linker.