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Chromosome landing at the barley Rar1 locus
T Lahaye1, K Shirasu, P Schulze-Lefert
1The Sainsbury Laboratory, John Innes Centre Norwich Research Park, UK.
Molecular & General Genetics : MGG
|November 26, 1998
Abstract:
The barley Rar1 gene is an essential component of the race-specific, Mla-12-specified powdery mildew resistance reaction. As part of a map-based cloning strategy designed to isolate Rar1, five barley yeast artificial chromosomes (YACs) have been identified, ranging in size from 300 to 1100 kb. PCR-based YAC end-specific markers have been established and were employed to construct a local YAC contig. Four out of five YAC clones were found to be non-colinear with the source DNA. High-resolution genetic mapping of the YAC ends demonstrated that the set of five overlapping YAC clones encompasses the barley Rar1 gene.