Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Pb(II)-translocating P-type ATPases

C Rensing1, Y Sun, B Mitra

  • 1Department of Biochemistry and Molecular Biology, Wayne State University, School of Medicine, Detroit, Michigan 48201, USA.

The Journal of Biological Chemistry
|November 26, 1998
PubMed
Summary

Staphylococcus aureus CadA and Escherichia coli ZntA are identified as lead(II) pumps, not just cadmium pumps. This finding expands our understanding of metal ion transport mechanisms in bacteria.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Measurement of the Positive Muon Anomalous Magnetic Moment to 127 ppb.

Physical review letters·2025
Same author

Measurement of the Positive Muon Anomalous Magnetic Moment to 0.20 ppm.

Physical review letters·2023
Same author

Purification, Crystallization, and Preliminary Crystallographic Studies of Human As(III) S-Adenosylmethionine Methyltransferase (hAS3MT).

Crystallography reports·2022
Same author

Haemodynamics as a determinant of need for pre-hospital application of a pelvic circumferential compression device in adult trauma patients.

Injury·2019
Same author

A checklist for trauma quality improvement meetings: A process improvement study.

Injury·2019
Same author

Video-tube thoracostomy in trauma resuscitation: A pilot study.

Injury·2018

Area of Science:

  • Microbiology
  • Biochemistry
  • Molecular Biology

Background:

  • The cad operon in Staphylococcus aureus confers cadmium resistance via CadA, an ATP-coupled Cd(II) pump.
  • Escherichia coli possesses a homologue, ZntA, also known as a Zn(II)/Cd(II) pump.
  • CadC acts as a metal-responsive repressor within the cad operon.

Purpose of the Study:

  • To investigate the potential of CadA and ZntA as lead(II) pumps.
  • To characterize the metal specificity of the CadC repressor.

Main Methods:

  • Assessing metal resistance conferred by CadA and ZntA.
  • Evaluating the effect of lead(II) on zinc(II) transport mediated by CadA and ZntA in everted membrane vesicles.
  • Determining the metal response profile of the CadC repressor.

Main Results:

  • CadC exhibits a metal response order of Pb(II) > Cd(II) > Zn(II).
  • Both CadA and ZntA confer resistance to lead(II).
  • Lead(II) inhibits the transport of zinc(II) catalyzed by CadA and ZntA.

Conclusions:

  • CadA and ZntA function as lead(II) pumps, expanding their known substrate range.
  • The CadC repressor's specificity suggests a broader role in regulating resistance to multiple soft metals.
  • These findings have implications for understanding bacterial metal homeostasis and resistance mechanisms.

Related Experiment Videos