Related Experiment Videos
Dialysis cultures
1Technische Universität Hamburg-Harburg, Bioprozess- und Bioverfahrenstechnik, Germany. maerkl@tu-harburg.de
Applied Microbiology and Biotechnology
|November 27, 1998
Summary
Dialysis fermentation effectively removes low-molecular-mass components, enabling high cell densities. This reliable technique avoids membrane clogging and optimizes nutrient use for superior microbial yields.
Area of Science:
- Biotechnology
- Biochemical Engineering
Background:
- High cell density fermentation is crucial for efficient bioprocessing.
- Traditional methods face challenges like membrane clogging and nutrient limitations.
Purpose of the Study:
- To evaluate dialysis techniques for achieving high cell densities in fermentation.
- To address scale-up challenges and optimize nutrient utilization.
Main Methods:
- Utilized membrane dialysis reactors with integrated and external loops.
- Employed a "nutrient-split" feeding strategy.
- Tested with various microorganisms including Staphylococci, E. coli, and Lactobacilli.
Main Results:
- Achieved cell densities up to 30 times higher than conventional methods.
- Demonstrated high efficiency and reliability without membrane clogging.
- Showcased efficient nutrient utilization and avoidance of medium optimization.
Conclusions:
- Dialysis is a highly effective and reliable method for high-density fermentation.
- The technique offers significant advantages in cell yield and process efficiency.
- Applicable across diverse microorganisms and scalable for industrial use.