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Use of capillary electrophoresis as a method development tool for classical gel electrophoresis
R M Sutton1, S R Gratz, A M Stalcup
1Department of Chemistry, University of Cincinnati, OH 45221-0172, USA.
The Analyst
|November 27, 1998
Abstract:
Capillary electrophoresis (CE) was used to optimize the buffer pH, ionic strength and sulfated cyclodextrin concentrations for enantiomeric separation of piperoxan. These enantioseparation conditions were then applied to a classical gel electrophoresis system. Binding constants of the sulfated beta-cyclodextrin-piperoxan couple were approximated using CE and the effects of organic solvents on the system were also investigated.