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Band broadening during dc and field inversion capillary electrophoresis of lambda DNA in dilute hydroxyethylcellulose
1Department of Chemistry, University of Michigan, Ann Arbor 48109, USA.
Abstract:
Capillary electrophoresis of lambda double-stranded (ds) DNA (48.5 kbp) in dilute hydroxyethylcellulose (HEC) solutions shows band spreading that cannot be explained by diffusion alone. Dispersion and asymmetry factors of lambda ds-DNA bands were measured as functions of capillary length, HEC concentration and field strength. Band spreading and asymmetry can be explained by a recently developed model in which the dominant contribution is assumed to be dispersion in DNA-HEC disentanglement times. Bandwidth reduction using square-wave field inversion was also investigated. It is proposed that correlation of DNA motion is the source of band narrowing during field inversion.