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Related Experiment Videos

[Genetic modification of the mouse]

K B Andersson1, B S Skålhegg

  • 1Universitetet i Oslo, Biokjemisk Institutt.

Tidsskrift for Den Norske Laegeforening : Tidsskrift for Praktisk Medicin, Ny Raekke
|November 27, 1998
PubMed
Summary
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Gene targeting, or knockout technology, enables precise genetic modification in mice for studying gene function. Advanced techniques like Cre/loxP allow sophisticated genetic engineering in whole animals.

Area of Science:

  • Molecular biology
  • Molecular medicine
  • Genetics

Context:

  • Homologous recombination enables precise gene mutation and study of consequences in intact animals.
  • A decade of advancements in gene targeting (knockout technology) in mice has been achieved.
  • Embryonic stem cell modification via homologous recombination is a foundational technique.

Purpose:

  • To review the historical and technical development of knockout technology.
  • To highlight applications in cancer biology, immunology, and neurobiology mouse models.
  • To discuss advancements in tissue- or time-specific genetic modifications.

Summary:

  • Gene targeting allows precise manipulation of single genes in mice, providing insights into their functions.
  • The article covers the evolution of knockout technology, its applications, and future directions.

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  • Cre/loxP systems combined with homologous recombination expand possibilities beyond simple gene inactivation.

    • Impact:

    • Facilitates in-depth study of gene function and disease mechanisms in vivo.
    • Accelerates research in fields like cancer, immunology, and neurobiology.
    • Enables sophisticated genetic engineering for a wide range of biological research applications.