Related Experiment Video
Updated: Oct 13, 2026

A Method to Assess Bacteriocin Effects on the Gut Microbiota of Mice
Published on: July 25, 2017
[Microwave effect on survival of sporulated bacteria inoculated in minced meat]
M L Arias1, M Jiménez, F Antillón
1Facultad de Microbiología, Universidad de Costa Rica.
Abstract:
Due to the current tendency of cooking and heating meat prepared foods in microwave ovens and the possibility that they transmit bacterial diseases, the survival rate of spore-forming bacteria was evaluated in minced meat samples. Meat was innoculated with a known number of Bacillus cereus and Clostridium perfringens spores, and laterly thawed and cooked in an Amana microwave oven (2450 Hz). Survival rate was determined according to the methodology described by Vanderzant & Splittstoesser, and the activity of the enzyme acid phosphatase was determined as cooking parameter. B. cereus spore showed a decrease in its number as the time of exposition increased, but without fully disappearing. C. perfringens spores also decreased in number, but showed a later increase, associated with the germination of survival spores.
Insights
Microwave cooking of minced meat reduces Bacillus cereus and Clostridium perfringens spores. Some C. perfringens spores survived and germinated, indicating potential bacterial risks from microwaved meats.
Area of Science:
- Food microbiology
- Bacterial spore survival
- Food safety
Context:
- Increasing popularity of microwave ovens for cooking and reheating meat products.
- Potential transmission of bacterial foodborne illnesses from inadequately prepared foods.
- Need to assess bacterial survival in microwaved minced meat.
Purpose:
- To evaluate the survival rate of Bacillus cereus and Clostridium perfringens spores in minced meat after microwave cooking.
- To determine the impact of microwave heating time on spore viability.
- To use acid phosphatase activity as a cooking parameter.
Summary:
- Minced meat inoculated with B. cereus and C. perfringens spores was thawed and cooked in a microwave oven.
- B. cereus spore counts decreased with increased microwave exposure time but did not eliminate.
- C. perfringens spore counts initially decreased but later increased due to germination of surviving spores.
Impact:
- Highlights the incomplete inactivation of certain spore-forming bacteria by microwave heating.
- Suggests potential public health risks associated with microwaved meat products.
- Underscores the importance of validated cooking parameters for food safety.
More Related Videos
10:03Investigating the Detrimental Effects of Low Pressure Plasma Sterilization on the Survival of Bacillus subtilis Spores Using Live Cell Microscopy
Published on: November 30, 2017
09:07The Effect of the Application of Thyme Essential Oil on Microbial Load During Meat Drying
Published on: March 14, 2018
Related Concept Videos
Microorganisms in Agriculture and Food industry
Physical Methods for Controlling Microbial Growth: Temperature
Microbes in Food Production
Microbial Spoilage of Food
Principles of Food Preservation
Methods of Controlling Food Spoilage