Related Experiment Videos
DNA copy number changes in childhood acute lymphoblastic leukemia
M L Larramendy1, T Huhta, K Heinonen
1Department of Medical Genetics, Haartman Institute, University of Helsinki, Finland.
Haematologica
|November 27, 1998
Summary
Comparative genomic hybridization (CGH) is a powerful tool for studying DNA copy number changes in childhood acute lymphoblastic leukemia (ALL). This study found CGH provides valuable additional information to standard cytogenetics, with a high success rate in ALL patient analysis.
Area of Science:
- Genomics
- Cancer Research
- Pediatric Oncology
Background:
- Comparative genomic hybridization (CGH) enables DNA copy number change analysis without cell culture.
- Previous CGH studies on childhood acute lymphoblastic leukemia (ALL) yielded discrepant results.
- This study analyzed 36 childhood ALL cases and compared findings with 157 prior cases.
Purpose of the Study:
- To analyze DNA copy number alterations in childhood ALL using CGH.
- To compare CGH results with existing literature.
- To evaluate CGH as a supplementary diagnostic tool for childhood ALL.
Main Methods:
- DNA extracted from bone marrow of 36 childhood ALL patients.
- Tumor and reference DNAs labeled and hybridized.
- Analysis performed using the ISIS digital image analysis system.
Main Results:
- Common chromosomal gains observed: X (42%), 10 (36%), 4 (31%), 6 (31%), 18 (33%), 14 (28%).
- Frequent chromosomal losses identified: 12p13-pter (14%) and 9p22-pter (6%).
- CGH showed a 100% success rate, outperforming conventional cytogenetics in some cases.
Conclusions:
- CGH revealed consistent patterns of DNA sequence gains across studies.
- Specific deletions (9p, 12p) were noted in this and one prior study.
- CGH offers significant additional diagnostic value to standard cytogenetics in childhood ALL.