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Related Experiment Videos

Collagen in tissue-engineered cartilage: types, structure, and crosslinks

J Riesle1, A P Hollander, R Langer

  • 1Division of Health Sciences and Technology, Massachusetts Institute of Technology, Cambridge 02139, USA.

Journal of Cellular Biochemistry
|November 27, 1998
PubMed
Summary

Engineered cartilage in vitro shows a collagen network organization and ultrastructure similar to natural articular cartilage. This suggests differentiated chondrocytes can form functional collagen matrices for tissue engineering applications.

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Area of Science:

  • Biomaterials Science
  • Tissue Engineering
  • Orthopedic Research

Background:

  • Articular cartilage's weight-bearing function relies on organized collagen types II and IX.
  • This collagen network balances the swelling pressure of the proteoglycan/water gel.
  • Assessing the functionality of engineered cartilage collagen networks is crucial for tissue engineering.

Purpose of the Study:

  • To determine if in vitro engineered cartilage contains a functional collagen network.
  • To analyze the composition and ultrastructure of collagen in engineered cartilage constructs.
  • To compare engineered cartilage collagen to natural articular cartilage.

Main Methods:

  • Chondrocyte-polymer constructs were cultured for up to 6 weeks.
  • Biochemical and immunochemical methods were used to analyze collagen composition.

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  • Scanning electron microscopy assessed collagen network ultrastructure.

    • Main Results:

    • Engineered cartilage had significantly lower total collagen content and pyridinium crosslinks compared to natural cartilage.
    • However, fractions of collagen types II, IX, and X were not significantly different.
    • Collagen network organization, density, and fibril diameter were comparable to natural articular cartilage.

    Conclusions:

    • Differentiated chondrocytes can form complex collagen matrices in vitro.
    • Engineered cartilage achieves a similar ultrastructural scale to natural articular cartilage.
    • These findings support the potential of tissue engineering for creating functional cartilage.