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Related Experiment Videos

Changes in hepatic polyamine catabolism in elderly rats

M E Ferioli1, A Sessa, E Rabellotti

  • 1Centro di Studio sulla Patologia Cellulare, C.N.R., Milan, Italy.

Liver
|November 27, 1998
PubMed
Summary

In aging rats, increased polyamine catabolism converts higher polyamines to putrescine. This age-related change may impair growth-related gene expression during senescence.

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Area of Science:

  • Biochemistry
  • Aging Research
  • Molecular Biology

Background:

  • Polyamines (putrescine, spermidine, spermine) are crucial for macromolecular synthesis, gene expression, and proteolysis.
  • Alterations in polyamine metabolism are implicated in cellular senescence.
  • Existing research primarily focuses on polyamine biosynthesis, leaving catabolism less explored.

Purpose of the Study:

  • To investigate the oxidative catabolism of polyamines in the liver of aging rats.
  • To understand the age-related changes in polyamine metabolic pathways.

Main Methods:

  • Measured polyamine oxidase (PAO) activity using N1-acetylspermine.
  • Assessed spermidine/spermine N1-acetyltransferase (SSAT) and diamine oxidase (DAO) activities using radiochemical methods.

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  • Quantified polyamines via HPLC with post-column derivatization.

    • Main Results:

    • SSAT activity increased in 36-month-old rats; PAO activity rose in 24- and 36-month-old rats.
    • Elderly rats showed decreased spermine and increased spermidine and putrescine, indicating enhanced interconversion of higher to lower polyamines.
    • DAO activity, which degrades putrescine, was elevated from 12 months of age.

    Conclusions:

    • Aged rat livers exhibit increased catabolic enzyme activity, leading to the reconversion of higher polyamines to putrescine.
    • This enhanced polyamine catabolism is a significant age-related alteration.
    • The observed changes may contribute to the impaired expression of growth-related genes in senescence.