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Related Experiment Videos

Analysis of acidic compounds using capillary electrochromatography

K D Altria1, N W Smith, C H Turnbull

  • 1Analytical Sciences, GlaxoWellcome R & D, Ware, Herts, UK.

Journal of Chromatography. B, Biomedical Sciences and Applications
|December 1, 1998
PubMed
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Capillary electrochromatography (CEC) offers a novel approach for separating acidic compounds. This review explores unique stationary phases, standard HPLC packings, and coated capillaries for diverse CEC applications.

Area of Science:

  • Analytical Chemistry
  • Separation Science

Background:

  • Capillary electrochromatography (CEC) merges capillary electrophoresis (CE) and high-performance liquid chromatography (HPLC).
  • CEC is gaining recognition as a complementary separation technique.

Purpose of the Study:

  • To review literature on the separation of acidic compounds using CEC.
  • To categorize CEC methods based on stationary phase types.

Main Methods:

  • Literature review of CEC separations for acidic compounds.
  • Categorization into novel/unique stationary phases, standard HPLC packings (e.g., ODS), and surface-coated capillaries.

Main Results:

  • Three main groups of CEC methods for acidic compounds were identified based on stationary phase characteristics.

Related Experiment Videos

  • Novel stationary phases, standard HPLC packings, and surface-coated capillaries are employed in CEC.
  • Various applications demonstrate CEC's separation capabilities and analytical performance.

    • Conclusions:

    • CEC is a versatile technique for separating acidic compounds.
    • The choice of stationary phase significantly influences CEC performance.
    • CEC shows promise for various analytical applications.