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Updated: Jul 31, 2026

Sheathless Capillary Electrophoresis–Mass Spectrometry for Metabolic Profiling of Biological Samples
Published on: October 1, 2016
Nonaqueous capillary electrophoresis with laser induced fluorescence detection
1Department of Chemistry, North Carolina State University, Raleigh 27695, USA.
Abstract:
This paper describes the use of nonaqueous capillary electrophoresis (NACE) with laser induced fluorescence (LIF) to improve detection sensitivity. The nonaqueous medium is conducive to lower detection limits due to the minimization of quenching effects. The nonaqueous solvent, N-methylformamide, produced the best detection limit with a 2-fold enhancement using NACE-LIF as compared to aqueous CE. N-methylformamide also gave the best fluorescent signal enhancement (6-fold) among the five nonaqueous solvents tested using steady-state fluorescence. The extent (or degree) of enhancement in fluorescence intensity seems to be related to the viscosity and/or polarity of the solvent.
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