Related Experiment Videos
New spectrophotometric procedure for determining cefotaxime based on derivatization with
L G Martinez1, P Campíns-Falcó, A Sevillano-Cabeza
1Departamento de Química Analítica, Facultad de Química, Universidad de Valencia, Burjassot/Valencia, Spain.
Abstract:
Cefotaxime was derivatised with 1,2-naphthoquinone-4-sulphonate (NQS), extracted into solid-phase cartridges (C18) and detected using a UV-visible detection system. Optimum conditions for this new procedure were: hydrogencarbonate-carbonate buffer, pH 10.5, 5-min reaction time at 25 degrees C and an NQS concentration of 7.1x10(-3) mol l(-1). The accuracy and the precision of the liquid-solid procedure were tested. The procedure was used to measure cefotaxime in pharmaceutical and urine samples. The results obtained were contrasted with those reported for a HPLC method for urine samples. The generalized H-point standard additions method was used to measure cefotaxime in urine samples.