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Related Experiment Videos

Mast cells express novel CD8 molecules that selectively modulate mediator secretion

T J Lin1, N Hirji, O Nohara

  • 1Pulmonary Research Group, Department of Medicine, University of Alberta, Edmonton, Canada.

Journal of Immunology (Baltimore, Md. : 1950)
|December 2, 1998
PubMed
Summary

Rat peritoneal mast cells (PMC) express functional CD8 molecules, distinct from those on T lymphocytes. This CD8 expression on PMC can induce tumor necrosis factor-alpha and nitric oxide production, but not histamine release.

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Area of Science:

  • Immunology
  • Cell Biology
  • Mast Cell Research

Background:

  • CD8 is a marker typically found on T lymphocytes and NK cells.
  • Mast cells are immune cells involved in allergic reactions and inflammation.
  • The presence and function of CD8 on mast cells are not well understood.

Purpose of the Study:

  • To investigate the expression and function of CD8 on rat peritoneal mast cells (PMC).
  • To determine if PMC CD8 molecules are similar to or distinct from those found on T lymphocytes.
  • To explore the functional consequences of CD8 engagement on PMC.

Main Methods:

  • Flow cytometry and confocal microscopy were used to detect CD8 expression on PMC.
  • In situ reverse-transcription PCR confirmed CD8alpha mRNA transcripts in PMC.

Related Experiment Videos

  • Functional assays measured TNF-alpha, nitric oxide, and histamine release upon CD8 antibody stimulation.

    • Main Results:

    • Freshly isolated rat PMC express both CD8alpha and CD8beta chains.
    • PMC CD8alpha appears distinct from T lymphocyte CD8alpha, as it does not bind the G28 antibody.
    • Engagement of CD8 on PMC induced TNF-alpha and nitric oxide production in a dose-dependent manner, but not histamine secretion.

    Conclusions:

    • Rat peritoneal mast cells express functional CD8 molecules.
    • The CD8 expressed on PMC may differ structurally from T cell CD8.
    • CD8 signaling in PMC appears to be independent of Fc epsilonRI signaling pathways.