Anoxia-induced changes in the resting potential of the cerebellar cells in tissue culture

Membrane potentials of Purkynĕ cells, granular cells, astrocyte cells and oligodendrocyte cells were measured in the cerebral tissue culture under normal conditions and after anoxia. An increment of the membrane potential value with age of culture was found. After anoxia the resting potential decreased with exception of 2-week Purkynĕ cells and granular cells. The highest anoxia-induced decrease of the membrane potential of all the cells studied was observed in 3-week tissue culture.