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RA70 is a src kinase-associated protein expressed ubiquitously
Y Kouroku1, A Soyama, E Fujita
1Division of Development and Differentiation, National Institute of Neuroscience, NCNP, Kodaira, Tokyo, 187-8502, Japan.
Biochemical and Biophysical Research Communications
|December 5, 1998
Summary
RA70, a protein homologous to SKAP55, is widely expressed across human tissues and cell lines. This adaptor protein interacts with src family kinases, suggesting a role in cellular signaling pathways.
Area of Science:
- Molecular Biology
- Cellular Signaling
Background:
- RA70 is a protein highly homologous to human src kinase-associated phosphoprotein (SKAP55).
- SKAP55 is known for its specific expression in thymus and T cells.
Purpose of the Study:
- To isolate and characterize the human full-length RA70 cDNA.
- To investigate the expression pattern and protein interactions of RA70.
Main Methods:
- Isolation of human full-length RA70 cDNA.
- Analysis of RA70 expression in various human tissues and cell lines using techniques like RT-PCR (implied).
- Assessment of RA70 interaction with src family kinases (Fyn, Hck, Lyn) via co-immunoprecipitation (implied).
Main Results:
- RA70 cDNA was isolated, revealing a protein highly homologous to SKAP55.
- RA70 exhibited ubiquitous expression in tissues (lung, skeletal muscle, spleen) and cell lines (U937), contrasting with SKAP55's restricted expression.
- RA70 was undetectable in thymus and Jurkat cells.
- RA70 (55 kDa) associated with the SH2 domain of Fyn.
- Interactions between RA70 and src family kinases (Fyn, Hck, Lyn) were observed during monocyte/macrophage differentiation of U937 cells.
Conclusions:
- RA70 functions as an adaptor protein for src family kinases, similar to SKAP55.
- RA70 likely plays a crucial role in the src signaling pathway across diverse cellular contexts.