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Updated: Aug 14, 2026

Mutagenesis and Functional Selection Protocols for Directed Evolution of Proteins in E. coli
Published on: March 16, 2011
Transcriptional mutagenesis and DNA strand asymmetrical mutations expressed in Escherichia coli under restrictive
1Department of Microbiology and Immunology, Indiana University School of Medicine, Indianapolis, IN 46023, USA.rbockra@iupui.edu
Abstract:
Mutagenesis by C-to-U events specifically in either DNA strand assayed with ung uvrA defective Escherichia coli on a metabolically restrictive medium produces more glutamine tRNA suppressor mutations from U occurring in the non-transcribed DNA strand than from U in the transcribed (template) DNA strand. This bias is the reverse of what might be expected from transcriptional mutagenesis (mutation expression utilizing mutated RNA transcribed from damaged template strand DNA). The results and related ideas are discussed.
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