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Tobacco BY-2 cells excise both 3-methyladenine and 7-methylguanine from methylated DNA
E Kraszewska1, M Dobrzańska, B Tudek
1Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Warsaw, Poland. elzbietak@ibbrain.ibb.waw.pl
Mutation Research
|December 5, 1998
Abstract:
Two of the major products in DNA resulting from exposure to alkylating agents are 7-methylguanine and 3-methyladenine. N-methylpurine-DNA-glycosylase is required for excision of these lesions. Recently, the 3-methyladenine-DNA-glycosylase gene of Arabidopsis thaliana was cloned and shown to be involved only in repair of 3-methyladenine. In BY-2 tobacco cells, we showed an enzymatic activity which excised both 3-methyladenine and 7-methylguanine from methylated DNA.