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Related Experiment Videos

White cell apoptosis in packed red cells

F Frabetti1, D Musiani, M Marini

  • 1Institute of Histology and General Embryology, University of Bologna, Italy.

Transfusion
|December 5, 1998
PubMed
Summary
This summary is machine-generated.

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Storage of red blood cells (RBCs) induces white blood cell apoptosis within 48-72 hours. This programmed cell death occurs regardless of anticoagulant or irradiation, impacting lymphocyte function and potentially transfusion reactions.

Area of Science:

  • Transfusion Medicine
  • Cellular Biology
  • Immunology

Background:

  • Packed red blood cell (RBC) units retain white blood cells (WBCs) post-buffy coat removal.
  • Storage conditions (1-6°C) can induce apoptosis in residual WBCs.
  • Understanding WBC apoptosis is crucial for evaluating febrile nonhemolytic transfusion reactions.

Purpose of the Study:

  • To evaluate the phenomenon of WBC apoptosis in stored RBC units.
  • To determine the influence of storage conditions on WBC apoptosis.
  • To assess the potential impact on transfusion reactions.

Main Methods:

  • Apoptosis in WBCs from 13 RBC units was assessed using microscopy, DNA electrophoresis, and cytometry.
  • RBC units were stored at 1-6°C, with samples analyzed periodically.

Related Experiment Videos

  • Lymphocyte proliferative capacity was measured post-phytohemagglutinin stimulation.

    • Main Results:

    • Apoptosis was observed in both granulocytes and lymphocytes within 48-72 hours of storage.
    • Anticoagulant-preservative solutions (CPD/SAGM vs. CPDA-1) and irradiation did not affect apoptosis.
    • Lymphocyte proliferative capacity significantly decreased with extended storage time.

    Conclusions:

    • Storage of RBCs consistently induces apoptosis in residual WBCs.
    • The role of apoptosis in biologic response modifiers and immune reactions requires further investigation.
    • Further research is needed to elucidate the clinical implications of WBC apoptosis in transfusion medicine.