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Semiquantitative bioluminescent assay of glutathione
F J Romero1, W Mueller-Klieser
1Institute for Physiology and Pathophysiology, University of Mainz, Germany.
Journal of Bioluminescence and Chemiluminescence
|December 5, 1998
Abstract:
A novel technique has been developed for semiquantitative detection of glutathione (GSH) in small volumes of liquid samples. GSH is detected via enzymatic linkage to the NADP/NADPH + H+ redox system through glutathione reductase. Accumulated NADPH is measured via the bioluminescent FMN oxidoreductase bacterial luciferase reaction. A linear correlation is obtained between bioluminescence intensity of the luciferase reaction and the GSH content of the liquid sample. Possible applications of this procedure are discussed.