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Characteristics of Staphylococcus aureus isolates from atopic dermatitis with reference to proteolytic activity
K Baran-Raunstrup1, J Miedzobrodzki, T Ternowitz
1Department of Dermatology, Marselisborg Hospital, University of Aarhus, Denmark.
Acta Microbiologica Polonica
|December 5, 1998
Summary
This study characterized Staphylococcus aureus strains from atopic dermatitis patients, finding most isolates produced proteolytic enzymes and haemolysins. Resistance to common antibiotics like ampicillin was prevalent, with no strain sensitive to all tested antimicrobials.
Area of Science:
- Microbiology
- Dermatology
- Infectious Diseases
Background:
- Staphylococcus aureus is a common pathogen in atopic dermatitis (AD).
- Understanding the biological diversity and antimicrobial resistance of S. aureus strains in AD is crucial for effective treatment.
Purpose of the Study:
- To investigate the biological properties, strain relationships, and antimicrobial susceptibility of Staphylococcus aureus isolates from patients with atopic dermatitis.
Main Methods:
- Biochemical typing using API Staph system.
- Phage typing.
- Analysis of proteolytic enzyme and haemolysin production.
- Antimicrobial susceptibility testing against fifteen antibiotics.
Main Results:
- Fifty-four S. aureus strains were classified into seven biotypes and three phage groups, with biotype A and phage group III being most common.
- All isolates produced proteolytic enzymes (87.0% high/moderate activity), and 83.0% produced alpha or beta haemolysins.
- High sensitivity was observed for chloramphenicol, neomycin, and fucidin, while resistance was high for ampicillin, oxacillin, and rondomycin. No isolate was sensitive to all antibiotics.
Conclusions:
- Staphylococcus aureus strains from AD patients exhibit significant proteolytic and haemolytic activity.
- Widespread antibiotic resistance necessitates careful selection of antimicrobial agents for treating S. aureus infections in AD.
- No clear correlation was found between biochemical/phage types and antibiotic resistance patterns, highlighting the complexity of these bacterial populations.