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Dioctahedral smectite neutralization activity of Clostridium difficile and Bacteroides fragilis toxins in vitro
G Martirosian1, G Rouyan, T Zalewski
1Department of Medical Microbiology, Institute of Biostructure Warsaw Medical Academy, Poland.
Acta Microbiologica Polonica
|December 5, 1998
Abstract:
The neutralization activity of dioctahedral smectite for ten toxigenic Clostridium difficile and eight enterotoxigenic Bacteroides fragilis strains was studied using McCoy and HT 29/C1 cell lines, respectively. Minimalization of the cytopathic effect of C. difficile toxin B on McCoy cell lines by dioctahedral smectite dissolved in PBS was observed. After incubation with dioctahedral smectite the toxic effects of B. fragilis enterotoxins on HT/29C1 (human colon adenocarcinoma cell line) were eliminated. Best neutralization of B. fragilis toxin was achieved using dioctahedral smectite dissolved in BHI.