H+ISFET-based biosensor for determination of penicillin G

Area of Science:

• Biotechnology
• Biosensor Technology
• Enzyme-based Sensors

Background:

• Penicillin G is a crucial antibiotic with significant industrial production.
• Accurate and rapid quantification of Penicillin G is essential for fermentation process monitoring and quality control.
• Existing analytical methods can be time-consuming or require complex instrumentation.

Purpose of the Study:

• To develop and characterize a novel biosensor for the detection of Penicillin G.
• To evaluate the performance of the biosensor in terms of response time, linear range, precision, and stability.
• To assess the applicability of the biosensor for real-time monitoring of Penicillin G in fermentation samples.

Main Methods:

• Development of a biosensor integrating an H+ ion sensitive field-effect transistor (H+(-)ISFET) with immobilized penicillin G acylase.
• Characterization of sensor performance using varying concentrations of Penicillin G in a phosphate buffer.
• Validation of the biosensor against the High-Performance Liquid Chromatography (HPLC) method using fermentation samples.

Main Results:

• The developed biosensor exhibited a rapid response time of 30 seconds for Penicillin G detection.
• A linear calibration range from 0.5 to 8 mM was achieved at pH 7.0.
• The sensor demonstrated excellent precision (coefficient of variation < 5%) and long-term stability (approx. 6 months, >1000 runs).
• Correlation analysis showed high agreement with HPLC (r = 0.9944), confirming its suitability for fermentation monitoring.

Conclusions:

• The H+(-)ISFET-based biosensor provides a sensitive, rapid, and stable platform for Penicillin G quantification.
• This biosensor is a viable alternative to conventional methods for monitoring Penicillin G in industrial fermentation.
• The technology holds potential for real-time process control and optimization in antibiotic production.