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Related Experiment Videos

Cyanide determination by an ISFET-based peroxidase biosensor

V Volotovsky1, N Kim

  • 1Institute of Molecular Biology and Genetics, NASU, Ukraine.

Biosensors & Bioelectronics
|December 5, 1998
PubMed
Summary

This study developed a biosensor using horseradish peroxidase (HRP) and an Ion Sensitive Field Effect Transistor (ISFET) for cyanide detection. Immobilization methods significantly impacted sensitivity, with a polymer matrix enhancing detection limits for cyanide ions in water.

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Area of Science:

  • Biosensors
  • Electrochemistry
  • Environmental Monitoring

Background:

  • Horseradish peroxidase (HRP) is a versatile enzyme for electrochemical sensing applications.
  • Ion Sensitive Field Effect Transistors (ISFETs) offer a platform for developing sensitive biosensors.
  • Cyanide detection in aquatic media is crucial for environmental and industrial safety.

Purpose of the Study:

  • To investigate the performance of HRP-immobilized ISFETs for cyanide ion determination.
  • To compare the effect of different immobilization matrices (BSA gel vs. PVPy polymer) on sensor sensitivity and stability.
  • To evaluate the reversibility of enzyme inhibition and sensor reusability.

Main Methods:

  • Immobilization of horseradish peroxidase onto an ISFET surface using bovine serum albumin (BSA) gel.

Related Experiment Videos

  • Immobilization of HRP onto an ISFET surface using a positively charged polymer, poly(4-vinylpyridine-co-styrene) (PVPy).
  • Electrochemical measurements to determine cyanide ion concentrations and assess enzyme inhibition kinetics.

    • Main Results:

    • The BSA-immobilized HRP-ISFET sensor detected cyanide in the 10(-5)-10(-3) M range.
    • The PVPy-immobilized HRP-ISFET sensor exhibited enhanced sensitivity, detecting cyanide in the 10(-7)-10(-5) M range due to anion accumulation.
    • PVPy immobilization significantly lowered the KCN concentration causing 50% enzyme inactivation (0.6 microM vs. 80 microM for BSA).

    Conclusions:

    • Enzyme immobilization strategy critically influences the performance of HRP-ISFET biosensors for cyanide detection.
    • Polymeric matrices like PVPy can enhance sensor sensitivity and reduce the effective inhibitory concentration of cyanide.
    • The reversible nature of cyanide inhibition allows for sensor activity restoration via rewashing, enabling potential reuse.