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A Murine Model of Irreversible and Reversible Unilateral Ureteric Obstruction
Published on: December 20, 2014
In vitro ligation of ureters and urethra modulates fetal mouse bladder explants development
A Beauboeuf1, S Ordille, D R Erickson
1Department of Surgery, Hershey Medical Center, Hershey, PA 17033-0850, USA.
Tissue & Cell
|December 5, 1998
Summary
Bladder wall maturation involves collagen organization. Ligation of ureters and urethra in fetal mouse bladders promoted more orderly collagen packing, suggesting a role for intravesical pressure and growth factors.
Area of Science:
- Urology
- Developmental Biology
- Biomaterials Science
Background:
- Bladder compliance depends on collagen deposition and organization.
- Fetal bladder wall maturation is linked to urine accumulation.
- Fetal mouse bladders can mature in organ culture.
Purpose of the Study:
- To investigate how ureter and urethra ligation affects collagen organization in fetal mouse bladders during organ culture.
- To explore the role of intravesical pressure and luminal growth factors in bladder wall development.
Main Methods:
- Organ culture of fetal mouse bladders.
- Sirius red staining of bladder sections.
- Polarized light microscopy to analyze collagen fiber bundles.
- Ureter and urethra ligation as experimental manipulation.
Main Results:
- Ligation of both ureters and urethra led to more orderly collagen fiber bundle packing at the luminal edge of the lamina propria.
- Unligated bladder explants showed less organized collagen structures.
- These findings suggest ligation influences collagen organization during maturation.
Conclusions:
- Uretero-urethral ligation promotes organized collagen development in the fetal bladder wall.
- Increased intravesical pressure and/or luminal growth factor accumulation are proposed mechanisms.
- This study provides insights into the biomechanical development of the bladder.
