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Interleukin-5 modulates interleukin-8 secretion in eosinophilic inflammation
L H Faccioli1, A I Medeiros, A Malheiro
1Department of Parasitology, Microbiology and Immunology, School of Medicine of Ribeirão Preto, University of São Paulo, Brazil. facciol@fmrp.usp.br
Mediators of Inflammation
|December 5, 1998
Summary
Interleukin-5 (IL-5) is crucial for inducing eosinophilia in Toxocara canis infections. This study reveals IL-5 also modulates interleukin-8 (IL-8) secretion, impacting inflammatory responses.
Area of Science:
- Immunology
- Parasitology
- Cytokine Research
Background:
- Toxocara canis infection in guinea-pigs induces eosinophilia and elevates IL-8 levels.
- The precise role of IL-5 in modulating cytokine secretion during this parasitic infection is not fully understood.
Purpose of the Study:
- To investigate the dynamic changes in serum and bronchoalveolar lavage fluid (BALF) IL-8 levels during T. canis infection.
- To elucidate the role of IL-5 as a modulator of cytokine secretion, particularly IL-8, in this model.
Main Methods:
- Measurement of serum and BALF IL-8 levels in infected guinea-pigs over time.
- In vivo experiments using anti-IL-5 antibody treatment to assess its effect on eosinophilia and IL-8 levels.
- In vitro studies using lipopolysaccharide (LPS)-stimulated peritoneal cells treated with recombinant IL-5 (rIL-5) or anti-IL-5 antibody.
Main Results:
- Serum and BALF IL-8 levels mirrored the pattern of blood eosinophilia, increasing post-infection.
- Anti-IL-5 antibody treatment suppressed eosinophilia and increased IL-8 levels in vivo.
- In vitro, rIL-5 significantly inhibited IL-8 release from LPS-stimulated cells, an effect blocked by anti-IL-5 antibody.
Conclusions:
- IL-5 is fundamental for inducing blood eosinophilia in T. canis infection.
- IL-5 acts as a novel modulator of IL-8 secretion, influencing inflammatory cytokine dynamics.
- These findings highlight a new biological role for IL-5 in parasitic infections.