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Secretion of proteases from Pasteurella multocida isolates

E Negrete-Abascal1, V R Tenorio, M de la Garza

  • 1Departamento de Biología Celular, Centro de Investigación y de Estudios Avanzados del IPN. Apdo. Postal 14-740 México, D.F. 07000, Mexico.

Current Microbiology
|December 5, 1998
PubMed
Summary

Pasteurella multocida secretes neutral metalloproteases that degrade IgG. These proteases may be crucial for Pasteurella multocida

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Area of Science:

  • Microbiology
  • Veterinary Science
  • Biochemistry

Background:

  • Pasteurella multocida is an important animal pathogen.
  • Bacterial proteases play roles in pathogenesis and tissue colonization.
  • Characterization of P. multocida proteases is essential for understanding disease mechanisms.

Purpose of the Study:

  • To investigate the secretion and characteristics of proteases from P. multocida animal isolates.
  • To determine the enzymatic properties and potential pathogenic role of these proteases.

Main Methods:

  • Culturing of five P. multocida isolates from bovine, chicken, sheep, and pig.
  • Protease activity assays across a range of molecular masses and pH.
  • Inhibition and reactivation studies using chelating agents, protease inhibitors, and calcium.

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  • Degradation assays using IgG as a substrate.
  • Immunological detection of secreted proteins using antiserum against Actinobacillus pleuropneumoniae protease.

    • Main Results:

    • All P. multocida isolates secreted proteases with varying molecular masses.
    • Proteases exhibited optimal activity at neutral pH (6-7), were inhibited by chelating agents, and reactivated by calcium, suggesting they are neutral metalloproteases.
    • The secreted proteases degraded IgG.
    • Proteins secreted by P. multocida were immunologically related to Actinobacillus pleuropneumoniae protease.

    Conclusions:

    • Pasteurella multocida isolates produce and secrete neutral metalloproteases.
    • These proteases are capable of degrading IgG, a key component of the immune system.
    • Protease production is likely a significant factor in P. multocida's ability to colonize tissues and cause disease.