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[Isolation of anti-Cryptococcus neoformans immunoglobulin G]

M T Illnait Zaragozí1, G Martínez Machín, C Fernández Andreu

  • 1Instituto de Medicina Tropical Pedro Kourí.

Revista Cubana De Medicina Tropical
|December 8, 1998
PubMed

Two immunization schemes were applied to rabbits with an autoctonous strain aimed at obtaining an anti-Crytococcus neoformans IgG. Whole cells treated with formalin were used. Dosages and immunization routes were different. The sera obtained were titered and that with the highest titer was selected (1:1 024 by the laminar agglutination method) and purified by saline precipitation and ion exchange chromatography. An IgG of high purity and biological activity was obtained.

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