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Related Experiment Videos

Functional characterization of a Nup159p-containing nuclear pore subcomplex

N Belgareh1, C Snay-Hodge, F Pasteau

  • 1Centre National de la Recherche Scientifique, UMR144, Institut Curie, 75 248 Paris cedex 05, France.

Molecular Biology of the Cell
|December 8, 1998
PubMed
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Nup82p acts as a docking site for Nup159p and Nsp1p, essential for poly(A)+ RNA export. Loss of Nup159p from the nuclear pore complex (NPC) in nup82 mutants causes RNA export defects.

Area of Science:

  • Cell Biology
  • Molecular Biology
  • Biochemistry

Background:

  • Nup159p/Rat7p is a crucial FG-repeat nucleoporin at the cytoplasmic face of the nuclear pore complex (NPC).
  • It plays vital roles in poly(A)+ RNA export and NPC organization.
  • Previous studies established Nup159p's NPC anchoring via its carboxyl-terminal domain.

Purpose of the Study:

  • To investigate the interactions of Nup159p within the NPC.
  • To elucidate the role of Nup82p in the Nup159p/Nsp1p subcomplex.
  • To understand the molecular basis of poly(A)+ RNA export defects in nup82 mutants.

Main Methods:

  • Structural-functional analysis of Nup159p interactions.
  • Yeast genetics using nup82Delta108 and nup159-1/rat7-1 mutant strains.

Related Experiment Videos

  • Immunofluorescence microscopy to assess Nup159p localization.
  • In vivo transport assays for nuclear protein import and export.

    • Main Results:

    • Nup159p directly interacts with Nsp1p and Nup82p via its carboxyl-terminal domain.
    • A deletion in Nup82p (nup82Delta108) disrupts Nsp1p binding but not Nup159p-Nsp1p interaction.
    • Nup159p delocalizes from the NPC in nup82Delta108 cells at 37°C, correlating with Nup82Delta108p degradation.
    • Nup82p appears to function as a docking site for the Nup159p-Nsp1p complex.
    • Mutant strains showed minimal defects in nuclear protein import/export.

    Conclusions:

    • Nup82p is essential for anchoring Nup159p at the NPC.
    • The poly(A)+ RNA export defect in nup82 mutants is likely caused by Nup159p dissociation from the NPC.
    • This study clarifies the role of Nup82p in NPC structure and function, particularly in RNA export.