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Updated: Aug 7, 2026

Analysis of Nephron Composition and Function in the Adult Zebrafish Kidney
Published on: August 9, 2014
Kid-1 expression is high in differentiated renal proximal tubule cells and suppressed in cyst epithelia
R Witzgall1, N Obermüller, U Bölitz
1Institute of Anatomy and Cell Biology I, University of Heidelberg, 69120 Heidelberg, Germany.
Abstract:
The cDNA coding for the transcriptional repressor protein Kid-1 was cloned in a screen for zinc finger proteins, which are regulated during renal development and after renal ischemia. Kid-1 mRNA levels increase in the course of postnatal renal development and decrease after acute renal injury caused by ischemia or administration of folic acid. We have raised a monoclonal anti-Kid-1 antibody and demonstrate that the Kid-1 protein is strongly expressed in the proximal tubule of the adult rat kidney. During nephron development, the Kid-1 protein appears after the S-shaped body stage concomitantly with the brush-border enzyme alkaline phosphatase. In two animal models of polycystic kidney disease, the expression of Kid-1 is downregulated. The loss of expression of Kid-1 in cyst wall cells correlates with the loss of alkaline phosphatase histochemical staining. Kid-1 mRNA levels are also reduced in rodent renal cell carcinomas, another condition characterized by epithelial cell dedifferentiation and increased proliferation. We propose that Kid-1 plays an important role during the differentiation of the proximal tubule.
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