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Microtiter plate-format optode

S B Kim1, H C Cho, G S Cha

  • 1Department of Chemistry, Kwangwoon University, Seoul, Korea.

Analytical Chemistry
|December 9, 1998
PubMed
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New microtiter plate optodes offer rapid and reproducible electrolyte analysis in serum. These novel sensors provide high sample throughput and analytical performance comparable to existing clinical analyzers.

Area of Science:

  • Analytical Chemistry
  • Biomedical Engineering

Background:

  • Optodes are sensors that change optical properties in response to analytes.
  • Microtiter plate formats are widely used in high-throughput screening and diagnostics.

Purpose of the Study:

  • To develop and evaluate microtiter plate-format optodes for rapid electrolyte determination.
  • To optimize optode membrane composition for reproducible and fast response times.

Main Methods:

  • Assembling bulk-response membranes in 96-well plates via casting or screen printing.
  • Optimizing poly(vinyl chloride) (PVC) to plasticizer [bis-(2-ethylhexyl) sebacate (DOS)] ratios.
  • Incorporating neutral carriers (calixarene derivative for Na+, valinomycin for K+) and lipophilic pH indicator (ETH 5294).

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Main Results:

  • Optimized PVC:DOS ratio of 1:6 enabled equilibrium response within 5 minutes.
  • Demonstrated high sample throughput (approx. 100 samples in <5 min).
  • Achieved analytical performance comparable to potentiometric clinical analyzers for electrolyte determination in serum.

Conclusions:

  • Microtiter plate-format optodes are a viable tool for high-throughput electrolyte analysis.
  • These optodes offer rapid, reproducible, and clinically relevant results.
  • The platform provides additional electrolyte information from samples prepared for other assays.