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Related Experiment Videos

Evolutionary analysis of "hagfish amelogenin"

M Girondot1, S Delgado, M Laurin

  • 1URA 1137, Evolution et Adaptations des Systèmes Ostéo-musculaires, CNRS and Université Paris 7, France. mgi@ccr.jussieu.fr

The Anatomical Record
|December 9, 1998
PubMed
Summary

A hagfish cDNA sequence, claimed to be amelogenin, likely originated from rodent contamination. This finding challenges its significance for understanding the evolution of mineralized tissues.

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Area of Science:

  • Evolutionary biology
  • Molecular genetics
  • Paleontology

Background:

  • Hagfishes are jawless fish that lack mineralized tissues and teeth.
  • A cDNA sequence, purportedly hagfish amelogenin, was recently cloned, potentially impacting views on mineralized tissue evolution.
  • Phylogenetic analysis of this cDNA sequence was previously absent.

Purpose of the Study:

  • To conduct a phylogenetic analysis of the cloned hagfish cDNA sequence.
  • To determine the evolutionary origin of the cloned hagfish cDNA sequence.
  • To assess the validity of the cloned sequence as hagfish amelogenin.

Main Methods:

  • Phylogenetic analysis using both phenetic and cladistic methods.
  • Comparison of the hagfish cDNA sequence with known mammalian and non-mammalian sequences.

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  • Polymerase Chain Reaction (PCR) contamination assessment.

    • Main Results:

    • The amplified hagfish cDNA sequence did not align with a non-mammalian origin.
    • The sequence showed a strong resemblance to a degraded rodent sequence.
    • Evidence suggests mammalian origin, likely due to PCR contamination.

    Conclusions:

    • The cloned hagfish cDNA sequence is unlikely to be of hagfish origin.
    • The sequence is probably a contaminant from a mammalian (rodent) source.
    • The finding questions the relevance of this cloned sequence for studying the evolution of mineralized tissues in hagfish.