Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Enzyme polymorphism in Fasciola hepatica L.: esterases

H A Alcaino, N F Baker, R A Fisk

    American Journal of Veterinary Research
    |October 1, 1976
    PubMed
    Summary

    This study identified seven distinct esterase isozymes in Fasciola hepatica from sheep bile ducts using isoelectric focusing. Isozyme patterns varied among individual flukes and local populations, indicating genetic diversity in Fasciola hepatica.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Seasonal occurrence of infective nematode larvae in California high Sierra pastures grazed by cattle.

    American journal of veterinary research·2013
    Same author

    Immunodiagnosis of fasciolosis in horses and pigs using western blots.

    International journal for parasitology·1998
    Same author

    Wanted: timely receipt of drug-recall information.

    American journal of health-system pharmacy : AJHP : official journal of the American Society of Health-System Pharmacists·1995
    Same author

    Strategic use of anthelmintics to prevent parasitic gastroenteritis in cow-calf herds in California.

    American journal of veterinary research·1990
    Same author

    Levels of serum IgE in response to gastrointestinal nematodes in cattle.

    Veterinary parasitology·1989
    Same author

    Seasonal prevalence of gastrointestinal nematodes of beef calves grazed on irrigated pastures in the lower Sacramento Valley of California.

    American journal of veterinary research·1988

    Area of Science:

    • Parasitology
    • Biochemistry
    • Molecular Biology

    Background:

    • Fasciola hepatica is a significant liver fluke parasite affecting livestock.
    • Understanding the genetic diversity of parasite populations is crucial for developing effective control strategies.

    Purpose of the Study:

    • To characterize the esterase isozyme profiles of individual adult Fasciola hepatica.
    • To investigate the genetic variation within Fasciola hepatica populations using enzymatic markers.

    Main Methods:

    • Adult Fasciola hepatica were collected from sheep bile ducts.
    • Isozyme analysis was performed using isoelectric focusing in polyacrylamide flat-bed gels.
    • Zymograms were generated through enzymatic and staining reactions to visualize esterase activity.

    Main Results:

    • Seven distinct alpha-naphthyl acetate hydrolyzing isozymes were identified with isoelectric points ranging from 5.10 to 5.75.
    • No single isozyme was present in all examined Fasciola hepatica specimens.
    • Seven different phenotypes were observed, with varying frequencies across local parasite populations from different sheep origins.

    Conclusions:

    • Isoelectric focusing and zymography revealed significant allelic and genotypic variation in Fasciola hepatica esterases.
    • The observed phenotypic variation suggests genetic differentiation among Fasciola hepatica populations.
    • Further research is needed to explore other enzyme systems and their potential as markers for parasite population genetics.

    Related Experiment Videos