Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Search research articles

Related Experiment Videos

Transition metals as protease inhibitors

B Duffy¹, C Schwietert, A France

¹The Gibson Institute for Medical Research, Santa Rosa, CA, USA.

Biological Trace Element Research

|December 9, 1998

Summary

This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Baseline Neurocognitive Functioning in Children/Teenagers and Young Adults Treated for Central Nervous System Malignancies Prior to Proton Therapy.

Clinical oncology (Royal College of Radiologists (Great Britain))·2026

Same author

The IMAP Observatory Overview.

Space science reviews·2026

Same author

The National Referral Service for Proton Beam Therapy in England: A Journey Towards Equitable Access.

Clinical oncology (Royal College of Radiologists (Great Britain))·2025

Same author

Assessing Equity of Access to Proton Beam Therapy: A Literature Review.

Clinical oncology (Royal College of Radiologists (Great Britain))·2023

Same author

Outcomes of Patients Treated in the UK Proton Overseas Programme: Central Nervous System Group.

Clinical oncology (Royal College of Radiologists (Great Britain))·2023

Same author

Outcomes of Patients Treated in the UK Proton Overseas Programme: Non-central Nervous System Group.

Clinical oncology (Royal College of Radiologists (Great Britain))·2023

Same journal

Distribution Patterns and Accumulation Factors of Rare Earth Elements in the Organism of Manchurian Wapiti and Wild Boar in Primorsky Krai, Russian Far East.

Biological trace element research·2026

Same journal

Biological Aging Mediates the Associations Between Multiple Serum Micronutrients and All-Cause Mortality Among U.S. Adults.

Biological trace element research·2026

Same journal

The Protective Effects of Selenium Nanoparticles Against Metal Toxicity.

Biological trace element research·2026

Same journal

Arsenic Exposure Induces Hepatic Fibrosis and Triggers Early Inflammatory Responses by Inhibiting Hepatic Stellate Cell PINK1/Parkin-Mediated Mitophagy.

Biological trace element research·2026

Same journal

Comparison of Mineral and Trace Element Contents in Commercial Zero-Labeled and Regular Non-Alcoholic Beverages.

Biological trace element research·2026

Same journal

How much Copper Do Calves Need? Retention and Hepatic Storage in Artificially Reared Milk-Fed Holstein-Friesian Calves.

Biological trace element research·2026

See all related articles

Transition metal ions, like titanium (IV), can inhibit serine proteases by directly binding to enzyme active sites. This novel approach shows promise for developing new antibacterial agents against common pathogens.

Area of Science:

Coordination chemistry
Enzyme inhibition
Antimicrobial development

Background:

Traditional protease inhibitors often use substrate analogs.
An alternative strategy involves using transition metal ions to block enzyme active sites.

Purpose of the Study:

To investigate the use of transition metal ion coordination chemistry for developing protease inhibitors.
To evaluate the efficacy of titanium (IV) as a protease inhibitor and its potential antimicrobial applications.

Main Methods:

Investigated the coordination chemistry of transition metal ions, specifically Ti(IV), with serine proteases (trypsin and chymotrypsin subclasses).
Assessed the binding mechanism and inhibition kinetics of Ti(IV) with trypsin.
Evaluated the effect of five-coordinate titanyl sulfate on the growth of Escherichia coli, Salmonella typhimurium, and Pseudomonas aeruginosa.

Related Experiment Videos

Main Results:

Aqueous Ti(IV) potently inhibited the trypsin subclass of serine proteases, but not the chymotrypsin subclass, via direct binding.
Direct binding was facilitated by specific enzyme active site features and the five-coordinate geometry of TiO(SO4)(H2O).
Five-coordinate titanyl sulfate completely inhibited the growth of E. coli, S. typhimurium, and P. aeruginosa.

Conclusions:

Direct metal ion binding offers an effective strategy for blocking enzyme active sites, potentially surpassing traditional organic inhibitors.
Five-coordinate titanyl sulfate demonstrates significant antimicrobial activity and may serve as a cost-effective alternative to conventional antibiotics for controlling specific bacterial infections.