Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Pax-8 protein levels regulate thyroglobulin gene expression

D Fabbro1, L Pellizzari, F Mercuri

  • 1Dipartimento di Scienze e Tecnologie Biomediche, Università di Udine, Piazzale Kolbe 1, 33100 Udine, Italy.

Journal of Molecular Endocrinology
|December 9, 1998
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Somatic genomic imbalances in 'tumour-free' surgical margins of oral cancer.

International journal of oral and maxillofacial surgery·2023
Same author

Tacrolimus Therapeutic Drug Monitoring in Stable Kidney Transplantation and Individuation of CYP3A5 Genotype.

Transplantation proceedings·2019
Same author

Parchment processing and analysis: Ionizing radiation treatment by the REX source and multidisciplinary approach characterization.

Applied radiation and isotopes : including data, instrumentation and methods for use in agriculture, industry and medicine·2019
Same author

Communication: Optically induced decoupling of the smectic-A and nematic order parameters at the AN phase transition in phototropic liquid crystals.

The Journal of chemical physics·2018
Same author

Cataract and optic disk drusen in a patient with glycogenosis and di George syndrome: clinical and molecular report.

BMC ophthalmology·2017
Same author

Simultaneous specific heat, thermal conductivity and imaging evaluations in thin samples of 8CB liquid crystal dispersed with microemulsion of DDAB/water micelles.

The Journal of chemical physics·2016
Same journal

A Computational Systems Biology View on the Role of the Menstrual Cycle in Endocrine Health and Disease.

Journal of molecular endocrinology·2026
Same journal

Pancreatic β-cell aging in physiology and diabetes: emerging roles of m6A mRNA methylation.

Journal of molecular endocrinology·2026
Same journal

Neuroendocrine regulation of female fertility: the role of CNS-derived hormones.

Journal of molecular endocrinology·2026
Same journal

Hypothalamic neuropeptides as modulators of neural activity and behaviour.

Journal of molecular endocrinology·2026
Same journal

Massively parallel functional genomic assays in endocrinology: from promise to delivery.

Journal of molecular endocrinology·2026
Same journal

TSH promotes chemerin/CMKLR1-cAMP/ERK-DIO2 signaling in primary rat ependymal cells in vitro.

Journal of molecular endocrinology·2026
See all related articles

Pax-8 protein levels regulate thyroglobulin gene transcription in thyroid cells. This transcription factor

Area of Science:

  • Molecular Biology
  • Endocrinology
  • Genetics

Background:

  • Pax proteins are crucial transcription factors governing cell differentiation.
  • Pax-8 is specifically expressed in adult follicular thyroid cells, interacting with thyroglobulin and thyroperoxidase gene promoters.

Purpose of the Study:

  • To investigate the role of Pax-8 protein levels in regulating thyroglobulin gene transcription.
  • To elucidate the mechanism by which Pax-8 influences thyroglobulin gene expression.

Main Methods:

  • Transfection of thyroid cells with a Pax-8 expression vector to increase protein levels.
  • Transfection with a thyroid transcription factor-1 (TTF-1) expression vector for comparative analysis.
  • Northern blot analysis of human papillary thyroid cancers.

Related Experiment Videos

  • Gel-retardation assays to study protein-DNA interactions.

    • Main Results:

    • Increased Pax-8 protein levels significantly enhanced thyroglobulin promoter activity.
    • TTF-1 expression vector transfection resulted in a modest decrease in thyroglobulin promoter activity.
    • A significant correlation was observed between Pax-8 and thyroglobulin mRNA levels in human papillary thyroid cancers.
    • Evidence suggests Pax-8 and TTF-1 compete for a common binding site on the thyroglobulin promoter.
    • Pax-8 expression is regulated by Thyroid Stimulating Hormone (TSH).

    Conclusions:

    • Pax-8 protein levels are a key regulator of thyroglobulin gene transcription.
    • Pax-8 may modulate thyroglobulin gene expression through competitive binding with TTF-1.
    • Pax-8 represents an important factor in the regulation of thyroid cell function, influenced by TSH.