Related Experiment Videos
[Molecular cloning and characterization of novel protein kinase gene DYRK3]
1National Laboratory of Medical Genetics of China, Hunan Medical University, Changsha, Hunan, 410078 P. R. China. nlmglcy@public.cs.hn.cn
Objective:
To isolate full length cDNA of a novel protein kinase and to deduce the protein kinase's classification position and functions.
Methods:
cDNA libraries gDNA library was screened with a partial cDNA clone which is homologous to human protein kinase DYRK2 as probe. FISH mapping was performed.
Results:
Two full length cDNAs of a novel protein kinase from human muscle cDNA library and human testis cDNA library were isolated. The full length cDNA from muscle has an open reading frame which is predicted to encode a protein of 588 amino acid residues and the cDNA from testis to encode a protein of 568 amino acid residues.
Conclusion:
Because the sequence from the 27th codon to the 3' end of the cDNA from muscle is identical to that from the 7th codon to the 3' end of the cDNA from testis, they should be different transcripts of the same gene. As the gene is highly homologous to human protein kinase DYRK2, the present authors termed the gene DYRK3. DYRK3 is homologous to many serine/threonine protein kinases such as yeast Yak1, human Clk1, human Mnb, drosophila melanogaster Mnb and Cdk2. DYRK3 should belong to the Clk family in CMGC group of serine/threonine protein kinase. DYRK3 has been mapped to chromosome 1q32 by FISH.