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Related Experiment Videos

Pathological and histological study on retinoblastoma

K Fujisawa1, K Itoh, Y Imai

  • 1Department of Pathology, Kobe University School of Medicine, Japan.

The Kobe Journal of Medical Sciences
|December 10, 1998
PubMed
Summary

This study classified retinoblastoma (Rb) into differentiated and undifferentiated types, finding distinct age distributions and histological features. Rb cell differentiation appears influenced by bcl-2 and Fas, but not BAX gene expression.

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Area of Science:

  • Ophthalmology
  • Oncology
  • Pathology

Background:

  • Retinoblastoma (Rb) is a pediatric ocular tumor.
  • Classification of Rb into differentiated (D-type) and undifferentiated (U-type) is based on histopathological features.
  • Understanding the molecular mechanisms underlying Rb differentiation is crucial for treatment.

Purpose of the Study:

  • To classify retinoblastoma cases based on histopathology and International Classification of Diseases for Oncology criteria.
  • To investigate the expression of proliferation and apoptosis markers in different Rb subtypes.
  • To explore the role of specific genes (bcl-2, Fas, BAX) in Rb cell differentiation.

Main Methods:

  • Pathological and histochemical analysis of 30 retinoblastoma (Rb) cases.

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  • Classification into D-type (18 cases) and U-type (12 cases).
  • Immunohistochemical staining for MIB-1, PCNA, bcl-2, Fas, BAX, NF, SP, and Tunel stains in 21 cases.

    • Main Results:

    • D-type Rb was more common in infants <1 year, while U-type was more frequent in aged infants (2-4 years).
    • D-type Rb exhibited more necrosis, degeneration, and calcification.
    • MIB-1 positivity correlated with increased necrotic areas. Bcl-2 and Fas were diffusely positive, BAX was negative, and Tunel staining showed few apoptotic cells.

    Conclusions:

    • Rb subtypes show distinct age and histological characteristics.
    • Cell proliferation marker MIB-1 correlates with tumor necrosis.
    • Rb cell differentiation may be modulated by bcl-2 and Fas, independent of BAX gene expression.