Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Reading DNA differently

Y Gat1, D G Lynn

  • 1G. D. Searle Chemistry Laboratory, University of Chicago, IL 60637, USA.

Biopolymers
|December 10, 1998
PubMed
Summary

Product inhibition hinders DNA ligation and polymerization. A new translation strategy overcomes this barrier, enabling information encoding and amplification into modified oligonucleotides.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Varicocele is the root cause of BPH: Destruction of the valves in the spermatic veins produces elevated pressure which diverts undiluted testosterone directly from the testes to the prostate.

Andrologia·2018
Same author

MEIOTIC ARREST IN OOCYTES REGULATED BY A SPISULA FACTOR.

The Biological bulletin·2018
Same author

Benign Prostatic Hyperplasia: Long-term follow-up of prostate volume reduction after sclerotherapy of the internal spermatic veins.

Andrologia·2017
Same author

The haustorium and the chemistry of host recognition in parasitic angiosperms.

Journal of chemical ecology·2013
Same author

Erect posture of humans leads to male infertility, BPH and prostate cancer.

Andrologia·2012
Same author

Chemical regulation of distance. Characterization of the first natural host germination stimulant for Striga asiatica.

Journal of the American Chemical Society·2012

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Synthetic Biology

Background:

  • Product inhibition is a major limitation in template-directed ligation and polymerization reactions.
  • Overcoming this barrier is crucial for efficient nucleic acid synthesis and information transfer.

Purpose of the Study:

  • To review strategies for circumventing product inhibition in template-directed reactions.
  • To present a novel translation strategy that overcomes product inhibition.

Main Methods:

  • Review of existing literature on overcoming product inhibition.
  • Development and description of a translation strategy for DNA amplification.

Main Results:

  • Identified product inhibition as a key bottleneck in template-directed synthesis.
  • Demonstrated a translation strategy that successfully overcomes product inhibition.
  • Enabled amplification of information encoded in DNA into backbone-modified oligonucleotides.

Conclusions:

  • The developed translation strategy effectively resolves product inhibition.
  • This approach allows for the faithful reading and amplification of genetic information.
  • Opens new avenues for synthesizing modified oligonucleotides for various applications.

Related Experiment Videos