Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Ribozyme chemogenetics

S A Strobel1

  • 1Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, CT 06520, USA. strobel@csb.yale.edu

Biopolymers
|December 10, 1998
PubMed
Summary

Chemogenetics reveals the chemical underpinnings of large ribozyme structure and function. This approach, using nucleotide analogues and chemical suppressors, has elucidated key tertiary hydrogen bonds in the Tetrahymena group I ribozyme.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Linguistic analysis of project ownership for undergraduate research experiences.

CBE life sciences education·2012
Same author

Science education. Changing the culture of science education at research universities.

Science (New York, N.Y.)·2011
Same author

Amylin replacement with pramlintide as an adjunct to insulin therapy improves long-term glycaemic and weight control in Type 1 diabetes mellitus: a 1-year, randomized controlled trial.

Diabetic medicine : a journal of the British Diabetic Association·2004
Same author

Exploring the mechanism of the peptidyl transfer reaction by chemical footprinting.

Cold Spring Harbor symposia on quantitative biology·2003
Same author

Biochemical identification of A-minor motifs within RNA tertiary structure by interference analysis.

Biochemical Society transactions·2002
Same author

The human amylin analog, pramlintide, corrects postprandial hyperglucagonemia in patients with type 1 diabetes.

Metabolism: clinical and experimental·2002

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Structural Biology

Background:

  • Chemogenetics, initially for DNA-protein interactions, now applies to RNA.
  • Large catalytic RNAs (ribozymes) require detailed structural and functional analysis.

Purpose of the Study:

  • To review chemogenetic methods for understanding ribozyme chemical structure and function.
  • To highlight the analogy between chemical modifications and genetic mutations in RNA.

Main Methods:

  • Utilizing nucleotide analogues for single-site substitution and interference mapping.
  • Investigating chemical revertants and suppressors analogous to genetic ones.
  • Focusing on the Tetrahymena group I ribozyme as a model system.

Main Results:

  • Defined an ensemble of tertiary hydrogen bonds crucial for ribozyme catalysis.
  • Developed a detailed model of the ribozyme catalytic core.
  • Identified a metal ion binding site, a helix-helix packing motif, and a triple helix interaction.

Conclusions:

  • Chemogenetic approaches provide powerful insights into ribozyme structure-function relationships.
  • The established model offers a framework for further investigation of ribozyme mechanisms.
  • This methodology advances the understanding of complex catalytic RNA molecules.

Related Experiment Videos