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Thyroid hormone stimulates progesterone release from human luteal cells by generating a proteinaceous factor
1Department of Zoology, Visva-Bharati University, West Bengal, India.
The Journal of Endocrinology
|December 10, 1998
Summary
Thyroid hormone (T3) stimulates progesterone secretion in women by activating a protein factor. This protein, conserved across species, mediates T3
Area of Science:
- Endocrinology
- Reproductive Biology
- Cell Biology
Background:
- Thyroid hormone deficiency is linked to reduced progesterone secretion during the luteal phase.
- The precise mechanism of thyroid hormone action on human luteal cells remains unclear.
Purpose of the Study:
- To investigate the effect of thyroid hormone on progesterone production in human luteal cells.
- To identify potential mediators of thyroid hormone action in the ovary.
Main Methods:
- In vitro incubation of human luteal cells with 3,5,3'-triiodothyronine (T3).
- Assessment of progesterone release and protein synthesis.
- Isolation and testing of a thyroid hormone-induced factor (TIF) and purified thyroid hormone-induced protein (TIP).
Main Results:
- T3 significantly stimulated progesterone release and protein synthesis in luteal cells.
- The T3 effect was blocked by cycloheximide and anti-T3 antibody, indicating a protein mediator.
- A partially purified factor (TIF) and purified TIP from other species also stimulated progesterone release, confirming a proteinaceous and conserved mediator.
Conclusions:
- Thyroid hormone (T3) stimulates progesterone release from human luteal cells via a protein mediator.
- This mediator, a thyroid hormone-induced protein, appears to be evolutionarily conserved.
- Findings suggest an indirect mechanism for T3 action on ovarian luteal function.