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HUGE: a database for human large proteins identified by Kazusa cDNA sequencing project
1Kazusa DNA Research Institute, 1532-3 Yana, Kisarazu, Chiba 292-0812, Japan.
Nucleic Acids Research
|December 10, 1998
Summary
The Human Unidentified Gene-Encoded (HUGE) database predicts human large protein structures from large cDNA sequences. This resource aids in understanding novel human proteins and their functions.
Area of Science:
- Genomics
- Proteomics
- Bioinformatics
Background:
- Large human cDNAs (>4 kb) are a source of novel protein discovery.
- Identifying proteins encoded by these large cDNAs is crucial for understanding human biology.
Purpose of the Study:
- To establish the Human Unidentified Gene-Encoded (HUGE) database.
- To predict primary protein structures from large human cDNA sequences (>4 kb).
- To focus on cDNA clones encoding large proteins (>50 kDa).
Main Methods:
- Determination of over 700 human cDNA sequences (average size 5.1 kb).
- Data deposition into public databases.
- Prediction of protein primary structures from cDNA sequences.
Main Results:
- Generation of the HUGE database containing information on human large proteins.
- Identification of novel human protein candidates from large cDNA sequences.
- Over 700 human cDNA sequences made publicly available.
Conclusions:
- The HUGE database provides a valuable resource for researchers studying human large proteins.
- Predictive analysis of protein structures from large cDNAs advances functional genomics.
- The HUGE database facilitates further investigation into newly identified human genes and proteins.